摘要
目的 检测抽动障碍患儿巨细胞病毒DNA含量及T淋巴细胞亚群的临床意义。方法 采用荧光基因定量检测法分别对 101例抽动障碍患儿和 34例健康对照组儿童进行血清HCMV基因定量检测,并用间接免疫荧光染色法分别测定两组儿童外周血T淋巴细胞亚群。结果 抽动障碍患儿血清中HCMV检出率(36. 6% )显著高于正常对照组(2. 9% ) ( 2 =14. 07, P<0. 01),外周血CD4+、CD4+ /CD8+[ (36. 71±4. 67)%, (106. 76±19. 23)% ]较正常对照组 [ ( 40. 24±2. 51 )%, ( 131. 57±37. 69 )% ]显著降低,而CD8+ [ (35. 28±3. 96)% ]较对照组 [ ( 30. 63±7. 52 )% ]显著升高 (P<0. 01 )。抽动障碍患儿中HCMV阳性组CD8+ [ (37. 84±6. 93)% ]与阴性组[ (31. 45±6. 28)% ]比较显著升高,CD4+ /CD8+阳性组[ (90. 78±10. 76)% ]比阴性组[ (112. 76±14. 60)% ]显著降低(P<0. 01),而CD3+、CD4+两组间比较无显著差异(P>0. 05)。结论 HCMV感染可能是引起抽动障碍的一个重要原因。抽动障碍患儿存在明显的细胞免疫功能紊乱,表现为T淋巴细胞亚群平衡失调,提示细胞免疫功能紊乱可能与某些儿童易感HC MV及易患抽动障碍有关。
ObjectiveTo explore the clinical significance of human cytomegalovirus (HCMV) infection and T lymphocyte subgroups in children with tic disorder (TD). MethodsThe amount of HCMV-DNA in 101 patients with TD and 34 healthy children were done by fluorescent DNA ration detection, and the proportions of lymphocytic subsets were measured in two groups.ResultsThe positivism rate of HCMV-DNA of the patients with TD (36.6%) was obviously higher than that of the normal subjects(2.9%) ( 2=14.07,P<0.01), and the levels of CD4 +, CD4 +/CD8 +in children with TD were [(36.7±14.67)%, (106.76±19.23)%], which showed significantly depressed, as compared to that in healthy controls [(40.24±2.51)%, (131.57± 37.69)%]. The levels of CD8 + in TD [(35.28±3.96)%] were significantly increased, as compared to that in healthy controls [(30.23±7.52)%].ConclusionsThe infection of HCMV may be an important factor for TD. There was significant cell immune functional disorder in patient with TD and which may be related that some children have a higher rate of the infection of HCMV and TD.
出处
《中国行为医学科学》
CSCD
2005年第3期238-239,共2页
Chinese Journal of Behavioral Medical Science
