摘要
报道了不同碳源、维生素、氨基酸、钙盐及肌醇对新疆紫草悬浮培养细胞生长及紫草宁衍生物形成的影响。蔗糖是最适碳源、最佳浓度为３％。Ｂ族维生素对细胞生长及紫草宁衍生物形成的促进效果不大。酪氨酸以及甘氨酸会抑制产物的形成；而１０－５ｍｏｌ／ｌ的Ｌ－苯丙氨酸以及１０－７—１０－６ｍｏｌ／ｌ维生素Ｃ可明显提高紫草宁衍生物的含量及产量。肌醇对细胞生长的影响不大，但２００ｍｇ／ｌ肌醇可促进产物的形成。适于细胞生长及紫草宁衍生物形成的钙源分别为３３２ｍｇ／ｌＣａＣｌ２·２Ｈ２Ｏ和１４００ｍｇ／ｌＣａ（Ｎｏ３）２·４Ｈ２Ｏ。文末列出了改良的生长培养基及其配方。
A study on the effect of carbohydrates,vitamines,amino acids,calcium and inositol on the cell growth and shikonin derivative formation in suspension cultures of Arnebia euchroma cells was carried out.Sucrose is better than any other caybohytrates tested for cell growth and pigment formation,and the optimum concentration is 3%,Neither vitamine B2 nor vitamine B6 is effective for shikonin derivative formation,but vitamine B1 can promote cell growth.Both tyrosine and glycine retard the formation of shikonin derivatives,however,10-5mol/l L-phenylalanine and 10-7-10-6mol/l vitamine C stimulate their biosynthesis,Although inositol has no effect on cell growth,200mg/l inositol can increase pigment content.The optimal concentration of calcium salts for cell growth and shikonin derivative formation are 332mg/l CaCl2·2H2O and 1400mg/l Ca(NO3)2·4H2O,respectively,The hetter"growth medium"and"production medium"have been developed and listed in the present paper.
出处
《武汉植物学研究》
CSCD
1994年第4期348-354,共7页
Journal of Wuhan Botanical Research
关键词
新疆紫草
细胞悬浮培养
细胞生长
紫草宁衍生物
Arnebia euchroma
Cell suspension culture
Cell growth
Shikonin derivatives
Nutritional factors
