摘要
目的 比较不同来源的丹参中原儿茶醛的含量。方法 加热回流提取 ,采用高效液相色谱法 ,使用C 18柱 ,甲醇- 0 .0 5mol L枸橼酸溶液 -水 (18:2 0 :6 2 )为流动相 ,检测波长为 2 80nm。结果 方法线性关系良好 (r=1.0 0 0 ) ,平均回收率为96 .5 6 % ,RSD为 1.0 1% ,不同来源丹参中原儿茶醛的含量有显著性差异。结论 本法可有效地测定不同来源丹参中原儿茶醛的含量 ,方法准确可靠。
Objective To compare with the content of protocatechuic aldehyde in Salvia miltiorrhiza from different origin.Methods The protocatechuic aldehyde with heating and effluent extraction was extracted.The content of protocatechuic aldehyde was determined with HPLC.The fluid phase composed with acetic-0.05mol/L citric acid solution- water(18:20:62).The measuring wavelength was 280nm. Results The linearity of this measurement was distinct(r=1.000). The average reclaim rate was 96.56%,RSD was 1.01%.There was a marked difference about the content of the protocatechuic aldehyde among the difference source Salvia miltiorrhiza.Conclusions This measurement can determin the content of the protocatechuic aldehyde in Salvia miltiorrhiza from different origin.It is quite reliable.
出处
《食品与药品》
CAS
2005年第02A期46-49,共4页
Food and Drug
