摘要
本文介绍血清ＩｇＧ、ＩｇＡ、ＩｇＭ、Ｃ_３和Ｃ_４四种测定方法的方法学评价试验。结果表明：简易免疫比浊法，微量免疫比浊法和超微量免疫比浊法测定前述五项指标的准确度，精密度和灵敏度均高，且都比单向放射免疫扩散法好。并把原简易免疫比浊法中的反应时间从３７℃６０分钟改为３７℃３０分钟，其吸光度仍稳定；把原简易免疫比浊法中的测定波长从４９５ｎｍ改为３４０ｎｍ，原超微量免疫比浊法中的波长从４９２ｎｍ改为４５０ｎｍ，这样可以提高灵敏度。我们用这四种方法同时测定了６０份献血员血清ＩｇＧ、ＩｇＡ、ＩｇＭ、Ｃ_３和ｃ_４水平，并对这四种方法做了干扰试验。由于免疫比浊法操作简单、快速，且准确度、精密度和灵敏度均好，因此，其不失为测定血清ＩｇＧ、ＩｇＡ、ＩｇＭ、Ｃ_３和Ｃ_４较好的方法。
Four methods for measuring IgG,IgA,IgM,C3 and C4 in sera were evaluated.The results showed that the accuracy,precision and sensitivity of simple,micro-and ultramicro-immune turbidimetry for measuring the five items all are high and better than that of the single radial immunodiffusion method. The reaction temperature and time of the simple immune turbidimetry was changed from 37℃60 minutes to 37℃ 30 minutes,showing stable absorbance;for improving their sensitivities the wavelengths of the simple immune turbidinictryand the ultramicro-immune turbidimetry were changed from 495nm to 340nm and from 492nm to 450nm respectively.using these four methods, IgG, IgA,IgM,C3and C4 of 60 donors' sera were determined and interference tests also were done on all of them,The immune turbidimetry is simple,rapid ,accurate,precise and sensitive,and it is of choice for measuring Serum Igs,C3ang C4.
出处
《四川省卫生管理干部学院学报》
1994年第4期25-28,共4页
Journal of Sichuan Continuing Education College of Medical Sciences
关键词
IgG
IGA
IGM
C3
C4
血清诊断
免疫球蛋白
Immune turbidimetry
Single radial immunodiffusion
Methodologic evaluation
Immunoglobin,C_3and C_4 levels in serum.
