摘要
本文报告用蜡烛缸平皿法培养恶性疟原虫配子体，对云南分离的ＦｃｃＢＨ１／ＹＮ和ＦｃｃＭＤ１两株进行培养获得成功，两株分别在４ｄ和１ｄ出现配子体，１６ｄ和１０ｄ达高峰．最高密度分别为１３５和１２３／１００００ＲＢＣ，Ｖ期配子体最高密度为２０和３０／１００００ＲＢＣ，平均８．３和４．３／１００００ＲＢＣ．雌雄配子体比例为９：１和５：１，雄配子体可见出丝１—６条，表明配子体已达到成熟阶段，为国内首次报道．
Continuous culture of two isolates of Plasmodium falciparum collected from two malaria patientes in Yunnan was earned out with the established candle jar method. Young gametocyte first appeared on day 4 or 1 and the gametocytes peaked on day 16 or 10. The peak gametocytes parasitemia reached 135 and 123/10000 RBC respectively for two isolates. The highest density of matured gametocytes was 20 and 13/10000 RBC and the ratio between macrogamete and microgamete was 9: 1-5: 1. Rounded microgametes with 1-6 flagella were clearly seen indicating gametocytes attained to sexual maturity. But experimental infection of An. dirus and An. minimus with the viable cultured parasites was unsuccessful.
出处
《实用寄生虫病杂志》
1994年第2期30-31,共2页
Journal of Practical Parasitic Diseases
基金
云南省卫生厅资助
关键词
疟原虫
配子体
体外培养
Plasmodium falciparum, gametocyte, in vitro cultivation
