摘要
用光镜、电镜和生化分析方法研究DMSO在体外对MGC 80-3细胞的作用,不同浓度DMSO对MGC 80-3细胞有不同的生长抑制作用。1.5%DMSO是本研究较适合的浓度。1.5%DMSO处理7天后细胞生长率、分裂指数、克隆形成率和Con-A凝集率分别下降35.15%、18‰,90%和55.8%。处理后,癌细胞膜的碱性磷酸酶(不存在于正常人胃粘膜)比活性下降了90%,接种裸鼠致瘤率亦下降75%。处理后细胞主要形态变化是细胞变大,铺展,长直的微绒毛变成短突状,胞质内有发育特别好的高尔基体和丰富的粗面内质网,这些资料提示DMSO能改变MGC 80-3的恶性表型,对MGC 80-3细胞是一种诱导分化剂。
The in vitro effects of dimethyl sul-foxide (DMSO) on MGC 80-3 cells were studied by light microscopy, transmission electon microscopy and biochemical methods. The results indicated that the inhibitory effects of DMSO on the growth of MGC 80-3 cells was concentration dependent and 1.5% DMSO was suitable in the present study. The growth rate, mitotic index, colony forming efficiency and Con A-aggregation of the cells treated with 1.5% DMSO in vitro for 7 days was reduced respectively by 35.15%. 18‰. 90% and 55.8%. It was remarkable that the activity of membrance-associated alkaline phosphatase, which is not presented in
normal human gastric mucosa, was decreased by 90% in the treated cells. There was a 75% decrease of the rate of tutno-rigenesis in the treated cells as compared with the tumorigenic rate of the untreated MGC 80-3 cells inoculated into nude mice. Gross morphological changes of MGC 80-3 cells treated with 1.5% DMSO were evident including enlargement and flatness of the cells, stumpy microvillies instead of long and rigid ones, and extremely well-developed Golgi apparatus and rough en-doplasmic reticulum. This data indicated that DMSO was able to induce differentiation of MGC 80-3 cells and change their malignant phenotypes.
出处
《实验生物学报》
CSCD
1994年第3期282-287,共6页
Acta Biologiae Experimentalis Sinica
基金
国家自然科学基金部分资助
关键词
二甲亚砜
诱导
分化
胃肿瘤
腺癌
Human gastric adenocarcinoma. Differentiation. DMSO.
