摘要
手术或屠宰采取措囊胚（Ｂ）、扩张囊胚（ＥＢ）。孵出囊胚（ＨＢ），置于含１０％ＦＣＳ的ＧＩＴ液中进行４２℃水浴１０分钟的热应激和－７℃酒精糟１０分钟的低温感受试验。结果，热应激对猪胚的存活率及发育率均无影响（Ｐ＞０．０５）；而热应激提高了猪胚对低温的耐受程度，热应激的胚胎再经低温感受试验后，培养２４小时的ＥＢ（φ＜５０）、ＥＢ（φ≥５０）和ＨＢ的活细胞数分别较相应的对照组提高３５．５％、４１．３％和５１．１％，差异显著（Ｐ＜０．０５），但在热应激处理液中添加了蛋白合成抑制剂者没有产生这一效果，此结果证明，热应激很可能诱发猪胚内产生抗低温的应激蛋白质，这为进一步研究猪胚胎冷冻和改进猪胚冷冻前处理提供了依据和可能途径。
Porcine Blastocysts (B), expanded blastocysts (EB) and hatched blastocysts (HB) were colected by operation of slangier and exposed to heat shock. and cold Shock expriment. The results of this experiment were as follows: Heat shock did not affect the suvival and developed rate of porcine embryos (p>0.05). But it could increase tolerance of the porcine embryos to low temperature, for instanse,the alive cells of EB (φ<50, EB (φ≤50) and HB in the roup of Heat shock cultured for 24 his after cold shock increased by 35.5%, 41.3% and 51.5% (p<0.05) compering with control group. It was also found that. this effect could be inhibited by cycloheximde. It might be that the procine embryo treated by heat shock could produce some antistress-proteins to the secondary stress. There for the experiment has provided a possible way to improve pretreament of embryos freezingin in further studies.
出处
《生物技术》
CAS
CSCD
1994年第4期29-32,共4页
Biotechnology
关键词
猪胚
热应激
应激蛋白
耐低温性
Porcine embryo
Heat shock
Antistree-protein
Cryo-tolerance
