摘要
曲霉Ａ２８－８是一株优良的角蛋白酶分泌突变菌株。其最佳固体发酵培养基为：２０％羽毛粉，８０％麸皮和微量无机氮（每克培养基中加０．５ｍｇ）；最适产酶条件为：起始ｐＨ７．５～８．０，温度２８℃～３０℃，时间为６０～７０小时，酶活高达２５００ＫＵ／ｇ曲；最适酶解条件为ｐＨ７．０～９．０，温度４５℃～５０℃。
The selected Aspergillus mutant strain, A28-8, was an excellent keratinase-producing strain.The best solid state fermentation medium for producing keratinase consists of feather powder(20%),bran (80%) and inorganic nitrogen (0.5mg/g medium). The highest keratinase activity was about 2500KU/g under the optimum conditions of pH 7.5-8.0, 28℃-30℃ and 60-72 hours. The suitable enzyme-degrading conditions of pH 7.0-9.0 and 45℃-50℃ were also successfully achieved.
出处
《生物技术》
CAS
CSCD
1994年第4期11-14,共4页
Biotechnology
关键词
固态发酵
角蛋白酶
曲霉
Solid state fermentation
Keratinase
Aspergillus
