摘要
用多孔玻璃珠固定谷氨酸氧化酶与过氧化氢酶分别制成相应的固定化酶管,结合流动注射分析系统测定谷氨酸的含量。测定线性范围在0.1—2.0mmol/L,精度(C.V)0.7％,测定速率每小时80样以上,使用寿命至少4个月。在谷氨酸浓度低于2.5mmol/L时,pH在6.5—8.0,温度20—35℃,磷酸盐浓度在0.05—0.25mol/L范围内对测定几乎无影响,对不同发酵时间的谷氨酸发酵液测定,测定的结果与酶试剂盒及瓦氏法比较,结果一致,说明该方法已具有实际应用价值。
Two immobilized enzyme reactors, the glutamate oxidase and horseradish pe-rioxidase on glass beeds(120-20D mesh with 750-1000A pore size) were used in a flow injection system to determine the L-glutamate concentration. The linear relationship was ranged from 0. 1-2 mmol/L of glutamate. The reliability (C. V. ) and determination speed was 0. 7% and 60-80 samples/h, respectively. The reliable lifetime of this reactor was at least 120 days. If the L-glutamate concentration remain slower than 2. 5 mmol/L, there is almost no effects imposed by pH value temperature and phosphate as they are limited within the range of 6. 6-8. 0, 20- 35℃ and 0. 05-0. 25 mmol/L respectively. Different samples were taken at different stages during the whole fementation process and the L-glutamate concentration were determined comparatively by enzymetic kit Warburg's respiration apparatus and the immobilized enzyme reactors described in this paper. The results showed that the three techniques are comparable.
出处
《生物工程学报》
CAS
CSCD
北大核心
1994年第4期351-355,共5页
Chinese Journal of Biotechnology
关键词
谷氨酸
谷氨酸氧化酶
固定化酶
L-glutamate, L-glutamate oxidase, immobilised enzyme, flow injection analysis
