摘要
作者将霍乱弧菌O抗原及毒素B亚单位基因片段,经DNA体外重组技术,得到了能表达双价抗原的工程菌株1046(pMG305)。经GM1-ELISA分析表明该菌株能够表达特异的霍乱CT-B抗原,且能分泌到胞外,通过菌体凝集,全细胞O抗原酶联分析和血凝抑制试验表明在1046(pMG305)菌体表面表达了霍乱的O抗原,它的脂多糖O抗原通过SDS-PAGE电泳分析,显示它表达了霍乱LPS的特征区带。小鼠腹腔免疫后用霍乱弧菌毒株攻击表明,有良好的保护作用,因此1046(pMG305)可望成为霍乱活疫苗的候选株。
In this study, the engineered E. coli strain 1046 containing V. cholerae LPS-O and CT-B bivalent antigen genes has been successfully obtained by using DNA recom-binant techniques. E. coli 1046 (pMG305) could not only express CT-B antigen but also secrete CT-B into medium as shown by GM1-ELISA. Meanwhile, whole cell 0 antigen-ELISA, bacterialagglutination test and hemagglutination inhibition assay demonstrated that LPS-O antigen could be expressed on the cell surface by E. coli 1046(pMG305) and shown LPS bands specific for V. cholerae by SDS-PAGE assay. Mouse intraperitoneal immunization and challenge trial indicated that the E. coli 1046 (pMG305) provided good protection against virulent V. cholerae. The engineered strain reported here is expected to be a live oral vaccine candidate for V. cholerae.
出处
《生物工程学报》
CAS
CSCD
北大核心
1994年第4期289-294,共6页
Chinese Journal of Biotechnology
