摘要
研究神经节苷脂ＧＭ３（简称ＧＭ３）对部分纯化的人红细胞膜Ｃａ２＋－ＡＴＰ酶的作用和对完整兔红细胞浆［Ｃａ２＋］的影响。结果表明：外源性ＧＭ３对Ｃａ２＋－ＡＴＰ酶的作用呈浓度依赖性双相调节即浓度为１～６．５μｍｏｌ／Ｌ时抑制酶活性，浓度大于６．５μｍｏｌ／Ｌ时激活酶活性；ＧＭ３不影响钙调素（ＣａＭ）对酶的激活；ＣＭ３对兔红细胞浆［Ｃａ２＋］也呈浓度依赖性双相调节即在ＧＭ３浓度低于６９μｍｏｌ／Ｌ时［Ｃａ２＋］有不同程度升高，浓度大于６０μｍｏｌ／Ｌ时则降低［Ｃａ２＋］。
The effects of exogenous gangliosides GM3 on partially purified Ca2+-ATPase of human RBC and on cytosolic calcium of intact rabbit RBC were studied.Results reveal that: ① Exogenous GM3 Showed a concentration-dependent,biphasic effect on Ca2+-ATPase activity. Low concentrations (1～6.5μmol/L) of GM3 could inhibit Ca2+-ATPase while high concentrations of GM3(higher than 6.5 μmol/L) could activate the enzyme.GM3 was not able to reduce the stimulating effect of calmodulin on the enzyme. ② Exogenous GM3 was also showed biphasic function on cytosolic calcium concentration [Ca2+] in RBCs.When the concentration of GM3 was lower than 60μmol/L, [Ca2+] was increased to various degrees, but was decreased when GM3 concentrations were higher than 60μmol/L.
出处
《上海医科大学学报》
CSCD
1994年第5期385-387,共3页
Journal of Fudan University(Medical Science)
基金
国家自然科学基金
关键词
神经节苷脂
钙离子
腺苷三磷酸酶
ganglioside
Ca￣(2+)-ATPase
cytosolic free Ca￣(2+) concentration
