摘要
对７０例血清谷丙转氨酶（ＡＬＴ）异常、疑是丙型肝炎（ＨＣＶ）患者血清标本，用ＨＣＶ基因５＇非编码区引物作套式聚合酶链反应（套式ＰＣＲ），扩增产物为２６０ｂｐ，结果显示５２例丙型肝炎病毒核酸（ＨＣＶ－ＲＮＡ）阳性，１８例阴性，阳性率为７４．２％。１０例助血员血样ＨＣＶ－ＲＮＡ均为阴性。经Ｓｏｕｔｈｅｒｎｂｌｏｔ分析，阳性ＰＣＲ产物为ＨＣＶ－ＲＮＡ所特异。作者认为，采用高保守区核苷酸序列引物作套式ＰＣＲ检测ＨＣＶ－ＲＮＡ，可提高实验的敏感性和特异性。
We detected HCV-RNA with nested polymerase clain reaction using 2 pairs of primers deducde from 5’-noncoding region of HCV genome. The size of the amplifled fragments is 260 base pairs.Results showed that 52(74.2%) of 70 paitents suspected HCV infection with abnormal ALT were positive for HCV-RNA by the nested PCR, Overwheiling majority of patients(50 out of 52) positive for HCG-RNA were also positive for antibody to HCV by commercial assay kits.Serum samples from 10 donors were negative by both assays.Analyzed by Southernblot assay,the 260bp PCR products showed signs of hybridization.This suggested that HCV viremia can be more accurately diagnuosed by detecting the HCV-RNA with nested PCR using primers derived from highly conserved region,and both the sensitivity and specificity will be improved.
出处
《上海第二医科大学学报》
CSCD
1994年第3期195-198,共4页
Acta Universitatis Medicinalis Secondae Shanghai
