摘要
中压液相色谱纯化牛血红细胞超氧化物歧化酶韩庆宏，汪振珊，徐晓莹，孙琴美，陈福来，张乃忠（哈尔滨医科大学生化教研室哈尔滨１５００８６）１前言超氧化物歧化酶（ＳｕｐｅｒｏｘｉｄｅＤｉｓｍｕｔａｓｅＥＣ１．１５．１．１ＳＯＤ）广泛存在于生物体内。它能催化超...
A rapid and effective purification of superoxide dismutase from bovine erythrocytes by MPLC is repoted.After bovine erythrocytes were treated with chloroform-ethanol to remove hemoglobin,acetone was used toremove contaminant proteins.Then highly purified Cu-Zn SOD(the specific activity was 10 000～15 000U/mg pr.)was obtained by twice DEAE-Sepharose chromatography using different eluting procedures.SDS-PAGE of the Cu-Zn SOD purified as above revealed one band. The molecular weight was 1 5 000～17 000.The maximum ultraviolet absorption was at 264nm.
出处
《色谱》
CAS
CSCD
北大核心
1994年第1期67-69,共3页
Chinese Journal of Chromatography
关键词
牛
红细胞
超氧物歧化酶
色谱
medium pressure liquid chromatography,superoxide dismutase, erythrocyte
