摘要
取自肝癌高发区的40例人原发性肝细胞癌(PHC)标本,应用地高辛素(Dig)标记HBVDNA探针作原位杂交,检测PHC和癌周肝组织内HBVDNA的表达,并用光敏生物素C-myc癌基因探针原位杂交和用免疫组化检测HBsAg,HBcAg及P53癌基因在PHC和癌周肝组织内的表达。Dig-HBVDNA探针肝内总阳性率为80%,在PHC内为65%,而癌周肝组织中为73%。HBV-DNA定位于肝细胞或癌细胞的胞浆和胞核内,而HBVDNA阳性颗粒在胞浆内比胞核内多。HBVDNA阳性细胞癌周比癌内更多。结果表明,PHC病例与HBV存在关系密切;癌内HBVDNA大都属整合型,而癌周属游离型。研讨PHC内HBVDNA的表达与HBsAg,HBcAg,和P53及C-myc癌基因的表达相互关系及致癌作用。
Surgical specimens of 40 patients with human primary hepatocellular carcinoma(PHC) from the high-liver-cancer-incidence region were studied by in situ hybridization with Digoxigenin labelled probe for detecting HBV DNA and with photobiotin labelled probe for detecting expression of C-myc oncgene and by immunohistochemical staining for expression of HBsAg HBcAg and P53gene in PHC and pericancerous liver tissue respectively on formalin-fixed parafin-embedded section. The positive rate of HBV DNA was totally 80%(38/40)in the liver tissue, with 65%(26/40) in PHC tissuc and 73%(8/11) in the pericancerous liver tissue. HBV DNA was found mainly in cytoplasm of hepatocytes and cancer cells and more in the nucleus. More HBV DNA gunules were seen in the cytoplasmlsm than in nucleus. More HBV DNA positive cells were found in the pericancerous tissue than in cancerous tissue. These results showed that most of PHC cases selected were closey associated with the infection of HBV,HBV DNA in the pcricancerous tissue might be of the dissociate form, whie in the cancerous tissue it might be of the integrated form. The Expressive relationship between HBV DNA and HBsAg, UBcAg and C-myc gene,P53 gene in the liver and their pathogenesis are disscussed in this paper.
出处
《南通医学院学报》
1994年第1期1-4,共4页
ACTA Academiae Medicinae Nantong
关键词
乙型肝炎
抗原
原位杂交
肝细胞瘤
hepaitis B antigens
DNA viruses, primary hepatocelluar carcinoma
in situ hybridization