摘要
本文以粘红酵母ＲｈｏｄｏｔｏｒｕｌａｇｌｕｔｉｎｉｓＡＳ２．１０２，１００１－２Ｏ－６Ｒ和深红酵母ＲｈｏｄｏｔｏｒｕｌａｒｕｂｒａＡＳ２．２７９为出发菌株，经紫外诱变筛选得到一株苯丙氨酸解氨酶高活力菌株Ｒｈ．ｇｌｕｔｉｎｉｓＮＣ０６，其酶活力是亲株１００１－２０－６Ｒ的１．５倍。在最适转化条件下，肉桂酸转化率达５７．７％，每升转化液生成Ｌ－苯丙氨酸１１．５４克，另外对ＮＣ０６的生理特性进行了初步研究。
Three strain yeasts Rh. glutinis 1001-20-6R. AS2. 102 and Rh rubra AS2. 279 were mutagenised with UV irradiation. NC06,one of the mutants,exhibits 1. 5 times the phenylalanine ammonia lyase activity of parent 1001-20-6R. The optimum conversion condition was 2% the cinnamic acid,8mol/L. NH4OH, pH11. 0, and the reaction temperature was 30℃. Under the optimal conversion condition. L-phenylalanine was 11. 54g/L ,conversion yield for cinnamic acid was 57. 7%. In addition,physiology properties of NC06 were initial study.
出处
《南开大学学报（自然科学版）》
CAS
CSCD
1994年第4期31-36,共6页
Acta Scientiarum Naturalium Universitatis Nankaiensis
