摘要
用聚丙烯酸胺凝胶等电聚焦（ＩＥＦ）电泳对意蜂（Ａｐｉｓｍｅｌｌｉｆｅｒａ ｌｉｇｕｓｔｉｃａ）三个品系（浙农大Ａ系，湖北意峰和引进的原意蜂）的成年工蜂进行了苹果酸脱氢酶（ＭＤＨ）同工酶的测定。它们的ＭＤＨ图谱均有三个区带（ＭＤＨＩ、ＭＤＨⅡ和ＭＤＨⅢ），其中ＭＤＨⅡ是由三个等位基因（ａ、ｂ、ｃ）编码的，应有六种基因型（ａ／ａ、ｂ／ｂ、ｃ／ｃ、ａ／ｂ、ｂ／ｃ和ａ／ｃ），但本实验室测定的３６０余头个体中，均未出现ｂ／ｂ型．浙农大Ａ系出现了除ｂ／ｂ型以外的其它五种基因型，而其它两个品系都仅出现了ｃ／ｃ，ａ／ｃ和ｂ／ｃ等三种基因型。对结果进行独立性检验，浙农大Ａ系的ＭＤＨⅡ的基因型频率、基因频率以及杂合度等与其它两个品系均有很显著的差异，这说明它与这两个品系已有了相当程度的遗传分化，因此，这个结果可以为浙农大Ａ系的建立提供一个遗传和生化的依据。
he malate dehydrogenase (MDH) isozymes in three races of Apts melliferaligustica-Zhejiang Agricultural University A (A), Hubei A. m. ligustica (B) andA. m. ligustica introduced from italy (C) were analyzed with polyacrylamide gelisoele ctrofocusing (IEF). There were three zones (MDH I, MDH Ⅱ and MDH Ⅲ)in these electromorphs. MDH Ⅱ was coded by three alleies: a, b and c. About 120worker bees of each race were analyzed. Theoretically, there should be six genotypesin MDH Ⅱ (a/a, b/b, c/c, a/b, b/c and a/c), but b/b type was not found in theexperiment. The other five genotypes appeared in A, and only three (a/c. b/c andc/c) were found in B and C. Statistical analysis showed that there were significantdifferences in the genotype frequency, the allele frequency and the heterozygous degree between A and the other two races. This result provided a genetical and bioehemical evidence for the establishment of race A.
出处
《昆虫学报》
CAS
CSCD
北大核心
1994年第2期159-164,共6页
Acta Entomologica Sinica
关键词
意蜂
苹果酸脱氢酶
等位基因
基因
Apic mellifera ligustica -malate dehydrogenase-allozymes
