摘要
本文对从长江中获得的江豚进行了血液学、血液化学特征的测定和血红蛋白、血浆蛋白电泳图谱的研究。发现江豚的嗜中性粒细胞所占百分比较高,淋巴细胞的百分比较低,具有嗜碱性粒细胞。江豚血红蛋白的电泳类型为Ⅰ-Ⅱ,即电泳相对迁移率在人类Hb S和Hb F之间,并有4条非血红素蛋白带(NHP)。用醋酸纤维薄膜作支持物进行电泳,血浆的总蛋白(TP)带可分成8条主要的区带,从阳极到阴极依次是:前白蛋白(ζ)、白蛋白(A)和6条后白蛋白带(PA);然而用聚丙烯酰胺凝胶作支持物共可分成16条区带,其中14条后白蛋白带。脂蛋白(Lp)在聚丙烯酰胺凝胶上可分成4条区带:α_1-脂蛋白、α_2-脂蛋白和β-脂蛋白,分别相当于聚丙烯酰胺凝胶上总蛋白带电泳图谱中的PA9、PA10和PA11,而前-β-脂蛋白和乳糜微粒在起点处。运铁蛋白(Tf)的电泳图为一条区带,相当于PA6。
Hematology, Blood biochemical characteristics electrophoretograms of hemoglobin and serum protein of black finless porpoises from the Changjiang river were investigated. The percentages of granulocytes of neutrophils and lymphocytes are high. There are basophils on blood smears. Hemoglobin of black finless porpoises belongs to Hb type Ⅰ—Ⅱ, having the relative mobility rate of Hb between human Hb S and Hb F, with 4 bands of non-hem protein (NHP). By electrophoresis on cellulose acetate membrane, serum total protein (Tp) of the animal can be divided into 8 bands. From positive pole to negative pole, they are pre-albunin (ζ), albumin (∧) and 6 post-albumin (PA). By polyacrylamide gel, Tp presents 16 bands, among which lipoprotein has 4 bands: α_1-Lp, α_2-Lp, β-Lp band corresponding to PA 9, PA 10 and PA 11 respectively; pre-β-Lp and chylomicron are at the origin. Teansferrin presents one band, corresponding to PA 6.
出处
《水生生物学报》
CAS
CSCD
北大核心
1989年第1期76-82,共7页
Acta Hydrobiologica Sinica
关键词
江豚
血液学
Hematology, Blood biochemistry, Hemoglobin, Serum protein, Lipoprotein, Transferrin
