摘要
对流行程度不同的血吸虫病疫区人群，用ＭｃＡｂ－Ｄｏｔ－ＥＬＩＳＡ法检测循环抗原进行现场查病，同时与粪检虫卵和ＩＨＡ法查抗体相对照。结果，各不同流行区ＭｃＡｂ－Ｄｏｔ－ＥＬＩＳＡ法反应的阳性率和ＧＭＲＴ差异均与流行程度相吻合，从而能反映各流行区疫情状况。在重疫区，大多数受检者循环抗原测定结果与粪检及抗体测定结果相一致，符合率分别为７２．０９％和７１．５５％。在有重复感染机遇下，对治疗后一年人群进行复查，结果，循环抗原阴转快于抗体阴转。本实验表明：ＭｃＡｂ－Ｄｏｔ－ＥＬＩＳＡ和ＩＨＡ二法联合应用查病，具有敏感、特异、简便等优点，能较好地评估防治效果。
McAb-Dot-ELISA method was used to detect circulating antigen on site amongpopulation living in epidemic arca of schistosomiasis with a varying infectious degree. It was comparedwith stool sedimentation and hatching , and antibody determination by IHA. The results showed thatpositive rate of McAb-Dot-ELISA and GMRT difference matched with epidemic condition. Inseverely epidenic area, outcomes of circulating antigen determ ination for most patients also matchedwith stcol sedimentation and hatching and antibody determination. The coincidence rate were 72. 09%and 71. 55%respectively. The results of reexamination on patients who have been treated for one yearand under pOssible incidence of superinfection showed that antigen was faster to convert to be negativethan antibody.The study indicated that joint adoption of McAb-Dot-ELISA and IHA was a sensi-tive,specific and simple way to evaluate the prevention and cure of schistOsomiasis.
出处
《九江医学》
1994年第1期9-11,共3页
Jiujiang Medical Journal
关键词
血吸虫病
ELISA
循环抗原
McAb-Dot-ELISA method
circulating antigen
schistosomiasis Japonica
exami-nation on site
