摘要
给10头8周龄仔猪经口感染猪流行性腹泻肠管毒,于感染后5、15、25、35和45d各扑杀2头,采取胃及各段肠管标本,用免疫过氧化物酶技术(间接法)检查胃肠粘膜固有层中IgA、IgM和IgG产生细胞数;收集血液及胃、各段肠管分泌液,应用ELISA双抗体夹心法测定IgA、IgM和IgG含量。结果:实验猪感染后第15d,空肠下段、回肠和回盲口处粘膜固有层中IgA和IgM产生细胞明显增多,肠管分泌液中IgA含量与IgA产生细胞数呈正相关,肠道局部免疫反应的高峰比全身性(系统性)免疫出现得早,且周期短。本试验提示,肠道粘膜积极参与了该病的免疫过程;胃肠道对猪流行性腹泻病毒(PEDV)免疫反应的主要部位在空肠下段、回肠和回盲目;参与胃肠道免疫反应的免疫球蛋白产生细胞主要是IgA和IgM产生细胞。
Ten 8-week-old piglets were orally infected with porcineepizootic diarrhea virus(PEDV). Two piglets each were killed at 5,15, 25, 35 and 45d after infection. Two control piglets were killedat the same time. The immunoperoxidase technique was used todetermine the numbers of IgA-, IgM- and IgG-producing cells inintestinal mucosa. The direct ELISA was applied to find out thechanges of IgA, JgM and IgG in serum and gastrointestinal secretions.Results showed that the numbers of IgA- and IgM-producing cells inthe lower jejunum, ileum and ileocecal mouth mucosa of experimentalpigs infected for 15 days were greater than that of the control pigs.The IgA titer in the secretions of jejunum, ileum and ileocecal mouthwas directly related to the number of IgA-producing cells. The peakof gastrointestinal mucosal immune response was carlier than that ofsystemic immune reaction, but the duration of local mucosal immunitywas shorter than that of systemic immunity. The experiment resultsindicate that the mucosal tissues of intestinal tract participates inPEDJV immune reaction actively; the main immune response to PEDJVoccurs in the lower part of jejunum, ileum and ileocecal mouth; thecells that participate in gut immune response were mainly IgA-andIgM-producing cells.
关键词
猪
流行性腹泻
免疫
病理学
腹泻
porcine epizootic diarrhea
immunology
pathology
pig
