摘要
报道了用免疫酶法从人肝细胞cDNA文库中筛选清蛋白cDNA克隆的过程。所得到的克隆用免疫学方法及内切酶水解法进行鉴定。在7个阳性克隆中。
Using enzyme labeled immuno assay, the procedures of screening the clones of albumin cDNA from a library of human hepatic cells were reported. These selected clones were identified with immunological method and res- triction enzymes digestion, of the seven positive clones, the sum of three overlaps the whole length of coding region of albumin.
关键词
清蛋白
CDNA克隆
免疫酶法
血清
human serum albumin
cDNA clone
enzyme labeled immuno assay
E. Coli.
