摘要
本研究应用离体培养的兔卵巢颗粒细胞观察了ＩＦＮ－ａ对孕酮生成的影响。细胞预培养２４ｈ后，用ＩＦＮ－ａ（５０～８００Ｕ／ｍｌ）处理培养的颗粒细胞１２ｂ刺激孕酮的生成并呈剂量依赖关系。在ＩＦＮ－ａ加入培养细胞后３、１２和２４ｈ．刺激作用非常明显，但刺激作用的幅度在３和１２ｈ较大，分别为对照组的２４和２０倍。此外，ＩＦＮ－ａ刺激离体兔颗粒细胞孕酮生成的作用被同时加钙螫合剂ＥＧＴＡ（１ｍｍｏｌ／Ｌ）或钙通道阻断剂异搏定（ｖｅｒ）２０和２００μｍｏｌ／Ｌ所抑制。本研究结果表明，ＩＦＮ－ａ刺激离体兔卵巢颗粒细胞孕酮的生成，其作用依赖于细胞外的Ｃａ２＋浓度。
he present study was designed to examine the possible role of IFN-a in theregulation Of rabbit ovarian steroidogenesis,using isolated granulosa cells .Resultsshowed that IFN-a stimulated progesterone production in rabbit granulosa cells ina pose-and time-dependent manner and that the stimulatory effect of IFN-a wasblocked by condition of EGTA and verapamil.The results suggest that IFN-a playsa role in the regulation of rabbit ovarian steroidogenesis,via calcium dependent m-echanism.
出处
《基础医学与临床》
CSCD
1994年第3期30-32,共3页
Basic and Clinical Medicine
关键词
干扰素
兔
颗粒细胞
孕酮
a interferon rabbit granulosa cells progesterone Ca2+ * Department of Phaiology,Harbin Medical University ,Harbin
