摘要
合成了三种新荧光试剂,5—(4’—氯—2’—羧基苯偶氮)绕丹宁(CRACP,2)、5—(4’—溴—2’—羧基苯偶氮)绕丹宁(BRACP,3)及5—(4’—碘—2’—羧基苯偶氮)绕丹宁(IRACP,4).研究了它们的吸收光谱及荧光性质,探讨了分子结构与荧光性能的关系.建立了铜(Ⅱ)的荧光分析方法.在pH5.3时,CRACP与铜(Ⅱ)形成荧光螫合物,λ_(ex)/λ_(em)=310nm/408nm,检测限为5×10 ^(-4)μg/g.
Three new fluorescent reagents: 5-(4'-chloro-2'-carboxylphenylazo) rhodanine (CRACP), 5-(4'-bromo-2'-carboxylphenylazo) rhodanine (BRACP) and 5-(4'-iodo-2'-carboxylphenylazo) rhodanine (IRACP) were synthesized. Their absorbtion spectrum and fluorescence properties were studied. The influence of various environmental factors on fluorescence performance and the relation of molecular structure to fluorescence performance were also discussed. An analytical method of Cu (Ⅱ) based on the fluorescence of CRACP was established. CRACP reacts with copper (Ⅱ) to form a stable complex at pH 5.3, which shows an intense fluorescence at λex/λex = 310nm / 408nm. The detection limit of CRACP is 5×10-4μg ·g-1.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
1994年第12期1188-1193,共6页
Acta Chimica Sinica
基金
国家自然科学基金资助课题.
