摘要
本文报道一种新的蜂毒采集方法，测定了亚非马蜂（Ｐｏｌｉｓｔｅｓ ｏｌｉｖａｃｅｕｓ）粗毒的部分物理性状和生物学活性．该粗毒含有透明质酸酶、胆碱酯酶、蛋白水解酶、ＤＮＡ酶、酸性磷酸酶、碱性磷酸酶，并具有很强的溶血活性（其半溶血量ＨＵ_（５０）＝１７μｇ／ｍｌ），但肝素对其溶血作用有一定的抑制作用．其对小鼠ＬＤ_（５０）为５．５４ｍｇ／ｋｇ，对美洲蜚蠊ＬＤ_（５０）为７．４５μｇ／ｇ．药理学实验表明该粗毒对小鼠的膈神经－膈肌接头的神经冲动的传递以及膈肌的收缩都有不可逆的阻断作用．该粗毒能明显抑制蟾蜍离体心肌的收缩，但其抑制作用可被阿托品所解除。
A method to collect venom from the wasp (Polistes olivaceus) was reported.Partial physical and biochemical activities of the crude venom were analyzed. The LD_(50)of the venom in mice and cockroach were 5. 54mg/kg and 7. 45μg/g respectively. It showed enzyme activities of hyaluronidase, choline esterase, DNAse, protease, alkaline phosphetase and acid phosphatase. Its strong activity of haemolysis (HU_(50)= 17μg/ml) was observed and this activity can be inhibited by heparin to some extent. In the isolated mice phrenic nervediaphram preparation,the venom at a concentration of 5 × 10￣(-5)g/ml was found to block the neuromuscular transmission and the systole of diaphram irreversibly in 50 and 135 minutes respectively. In the isolated toad heart, the venom at a concentration of 1×10￣(-4)g/ml displayed an inhibition on the strength of the heart beat, which can be eliminated by atropine.
出处
《湖南师范大学自然科学学报》
CAS
1994年第3期49-54,共6页
Journal of Natural Science of Hunan Normal University
基金
湖南省教委资助
关键词
亚非马蜂
溶血性
生物活性
Polistes olivaceus
wasp venom :hyauluronidase
haemolysis
