摘要
利用去透明带金黄地鼠卵与人精子受精后,经过受精卵温育,制备出人精子单倍染色体,初期制片成功率47%。经过改善实验室条件和改进方法学,制片成功率达87%。本文还对一些技术上的关键问题进行了讨论。
By hybridization of Golden Hamster oocytes and human sperms and cultivation of fertilized eggs,the preparations of human sperm haploid chromosome were obtained.The success rate is about 87%.Some critical technical problems were discussed.
出处
《哈尔滨医科大学学报》
CAS
1994年第4期273-275,共3页
Journal of Harbin Medical University
关键词
人精子
受精
单倍
染色体
标本
Golden Hamster oocyte
Human sperm
Fertilisation
Haploid chromosome