摘要
本文通银杉(Cathaya argyrophylla Chun et Kuang)种子萌发的多因子正交试验[L_8(4×2~4)],提出了迄今为止银杉种子萌发的最佳方法,即用60mg/L IBA(吲噪丁酸)于20℃浸种18小时,然后在无菌条件下剥去种皮、切除部分胚乳(勿伤胚芽及胚根)并将种子竖直接种在MS琼脂培养基上,在20±1℃温度下萌发。种子萌发率达95%以上。
This paper has advanced the best method for seeds of Cathaya argyrophylla to germinate so far, through orthogonal test L_8 (4×2~4), that is,soak seeds in 60mg/L IBA solution 18 hour at 20℃. Then, in beacteria-free conditions,respectively remove seed coat, excise endosperms partially(can not injure plumule and radicle)and set the seeds upright On MS medium, and germinate them at 20±1℃. The germintion percent of the seeds was over 95%.
出处
《贵州大学学报（自然科学版）》
1994年第2期106-110,共5页
Journal of Guizhou University:Natural Sciences
关键词
银杉
种子
萌发
正交试验
松科
Cathaya argyrophylla
Seed germination
Orthogonal test
