摘要
考察了大孔苯乙烯－二乙烯苯交联共聚物的交联度、致孔剂量及胺化试剂对载体固定化猪胰脂肪酶的影响。选择出一种最佳载体对猪胰脂肪酶进行固定化，对比了自由酶和固定化酶在有机相中催化丁酸甲酯和正丁醇的酯交换反应。结果表明，酶经固定化后催化反应活力比自由酶提高近１倍。
Series of macroporous cross-linked polymers of styrene with divinylbenzene and their functionalization products were synthesized. Effects of degree of cross-linking,amount of pore-generating agent and different aminating agents on immobilization of porcine pancreas lipase(PPL)were studied.The best carrier was selected.Transesterification of methyl butyrate with 1-butanol was catalyzed by the free and immobilized PPL,it was found that the catalytic activity of immobilized PPL in organic systems was two times as high as that of the free PPL.
出处
《高等学校化学学报》
CSCD
北大核心
1994年第9期1335-1339,共5页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
关键词
猪胰脂肪酶
固定化
丁酸甲酯
酯化
Polystyrene
Porcine pancreas lipase
Immobilization
Organic systems
Transesterification
