摘要
化学发光酶联免疫分析检测血清中麻疹病毒抗体章竹君，邹克渭，程明洁（陕西师范大学分析科学研究所，西安，７１００６２）（陕西省卫生防疫站）关键词麻疹病毒抗体，化学发光，酶联免疫分析，辣根过氧化物酶目前在计划免疫工作中通常采用间接酶联免疫吸附分析法（ＥＬＩ...
A method for determination of the anti-MV antibody in serum is established based on chemiluminescence enzyme-linked immunosorbant assay (CL-ELISA). The MV antigen is coated on the polystyrene cup, then reacts with anti-MV serum to form the immunocompound. The anti-HuIgG labeled by HRP is used as a second antibody. The enzymatic activity on the antibody is determined by p-iodophenol enhanced luminol-H2O2 chemiluminescent system, which has higher sensitivity than current chromogenic reaction. The curve of anti-MV antibody title determined with CL-ELISA and ELISA is established and 30 clinical samples are tested. The results show that the CL-ELISA has higher positive ratio than ELISA. Two methods have good correlation. The correlation coefficient is 0.992.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1994年第4期515-517,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
关键词
麻疹病毒
抗体
ELISA
血清诊断
Anti-MV antibody, Chemiluminescence, Enzyme Immunoassay, HRP
