摘要
实验采用聚丙烯酰胺梯度胶电泳方法，对２６２尾黄鳝的性腺蛋白进行了分析，结果表明雌性黄鳝随着性腺的成熟性腺蛋白组分有所增多，相反雄性黄鳝随着性腺的成熟其性腺蛋白组分则有所减少；雌鱼性腺Ⅱ－Ⅲ期为１０－１２条区带，雄鱼性腺Ⅲ－Ⅵ期为１３－１０条区带。兼性期性腺蛋白组分明显增加为１５－１９Ｔ条区带，其增加的蛋白带为一组位于凝胶偏负极端、分子量相近的蛋白分子，性逆转时精巢的组建可能与这组蛋白的调节有关。性逆转从问时期卵巢开始不是一致的，其启动的时间可能受环境因素的调控；在兼性偏雌性性腺里的精原细胞可维持静止状态一段时期而不影响卵巢组织的正常发育，同样在兼性偏雄性的个体中其精巢可正常发育而不受残留的卵巢组织的影响。护巢亲鳝有全雄性和兼性偏雄性两种个体。
The gonadal protein of 262 individuals of Monopterus albus was analysed respecivcly through polyacrylamide gel (8%-15%, pH8.3) gradiented electrophoresis. The electrophoretic bands of gonadal Protein (10-12 protein bands) in female Monopterus albus (gonadal stage Ⅱ-Ⅲ) were increased with gonadal development but those (10-13 prote in bande) in male decreased at gonadal stage Ⅲ-Ⅵ. The gonadal protein bands of sex-reversing Monopterus albus have distincely increased (15-19 gonadal protein bands); the added protein bands, located the near negative pole, were similar in the molecular size and might be correlated to the male gonadal development during the sex-reversing stage. The female gonadal stage in which the sex change is started were variable, and the social environment (sex-ratio) of a given individual might be responsible for the trigger and transfor-mational process of the sex inversion. Spermatogonia might not be developed. and the ovary development would not be influenced in the ambisexual gonads of the active female phase, also the testis development might be ongoing and not he affected by the degonerated ovary in the ambisexual gonads of the active male phase. The male and functional male (ambisexual)guarded the nestes when the eggs of Monopterus albus were hatched.
出处
《动物学杂志》
CAS
CSCD
北大核心
1994年第1期15-17,共3页
Chinese Journal of Zoology
关键词
黄鳝属
分析
性逆转
性腺蛋白
Monopterus albus
sex reversal
gonadal protein
polyacrylamide gel gradiented electrophoresis.
