摘要
地被菊以茎尖为外植体进行组织培养，在附加ＢＡ３．０ｇｍ／Ｌ，ＮＡＡ０．０１ｍｇ／Ｌ成分的ＭＳ培养基上不定芽的增殖效果最好；在附加ＮＡＡ０．１ｍｇ／Ｌ成分的１／２ＭＳ培养基上生根效果最好。试管苗生长３～４ｃｍ高，每株生根达到５～７根，根系长０．５～１．０ｃｍ时，出瓶移栽入土效果好，成活率高；为降低成本改用３０％食用白糖代替蔗糖，其效果与蔗糖相同。
The tissue culture of Ground-coner Chrysanthemum with the tip of shoots as the explants was experimented. The results indicated that the multiplication of adventitious buds was best on Ms medium with the addition of BA 3.0 mg/1.NAA 0.01 mg/1;and the groWth of roots was best on 1/2 MS medium with the addition NAA 0.1 mg/1.It was better to trasplant from test-tube to soil when the plantlet in the test-tube grew to 3-4cm high and 5 or 7 rootlets of 0.5-1.0 cm long,then had the best percentage of survival.The cost could be lowered if 30 persent of edible powdered sugar was used to replace sucrose,hut the results were same.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
1994年第1期31-35,共5页
Journal of Northeast Forestry University
关键词
地被菊
繁殖
组织培养
Gwund-cover chrysanthemum group
Cytokinin
Auxin
Rapid propagation
