摘要
本文以氯化钾（ＣｅＣｌ＿３）沉淀的细胞化学方法，观察了水稻幼苗细胞在低温胁迫中质膜ＡＴＰ酶和５′－核苷酸酶活性的变化，得到的主要结果如下：（１）在适宜的生长温度（２８℃）下，水稻幼叶细胞内的ＡＴＰ酶主要定位于质膜，细胞间隙和胞间连丝等处，其中以质膜和细胞间隙处的酶活性最强；当幼苗经过１℃的低温处理后，质膜上的ＡＴＰ酶活性比适温生长时明显降低。（２）生长在适宜温度（２８℃）下的水稻幼苗，其细胞内的５－核苷酸酶活性专一性地定位于质膜和胞间连丝上，表现出很强的活性反应；幼苗在１℃低温处理２天后，其细胞内的５－核苷酸酶活性仍是严格定位于质膜及胞间连丝上，只是酶活性比低温处理前明显降低。这些结果进一步证明，质膜是低温伤害的最初部位。
In this paper,the activity changes of ATPase and 5-nucleotidase in the cells of the rice seedlings under low temperature stress were studied using cytochemical method of cerium trichloride precipitates。The main results are summarized as follows:(1) When rice seedlings grew at the optimum growth temperature(28℃), the ATPase in the cells of the rice seedlings was mainly localized in plasmolemma,plasmodesmus,and intercellular spaces,but only those AT-Pases, which situated in plasmolemma and intercellular spaces,mariifested high activities。When rice seedlings were treated at the temperature of 1 ℃ for 48 hrs,the ATPase activities in plasmolemma decreased considerably compared with those of the rice seedling growing at the optimum temperature。(2)When rice seedlings grew at the optimum temperature,the 5-nucleotidase in the cells of the rice seedlings uniquely located in plasmolemma and plasmodesmus,and showed high activities。The uniformly distributed deposits formed a black and clearly identifiable ring round the protoplasts;This indicates that the 5-nu-cleotidase,being used as an indicator of plasmolemma,is fit for not only animal materials,but plant materials also。When rice seedlings were treated at the temperature of 1℃ for 48 hrs,though the 5′-nucleotidase were still firmly localized in plasmolemma and plasmodesmi,its activities decreased significatly compared with those of non-treated rice seedlings,there only sparsely appeared some black deposits in plasmolemma for the treated seedlings。These results further indicate that plasmolemma is the primary site of low temperature stress。
出处
《电子显微学报》
CAS
CSCD
1994年第3期190-195,共6页
Journal of Chinese Electron Microscopy Society
