摘要
为了探讨糖皮质激发低亲和力结合部位（ＬＡＧＳ）介导大剂量糖皮质激素（ＧＣ）的效应，本文研究了氢化可的松（Ｆ）对原代培养大鼠肝细胞酪氨酸转氨酶（ＴＡＴ）诱导的量效关系。结果表明，Ｆ浓度在１—１０ｎｍｏｌ／Ｌ时，量效关系呈饱和趋势；超过１０ｎｍｏｌ／Ｌ直到１０μｍｏｌ／Ｌ，ＴＡＴ活性又不断上升，这种效应可被糖皮质激素受体（ＧＲ）的竞争性拮抗剂ＲＵ４８６完全阻断。提示ＬＡＧＳ也能介导Ｆ对ＴＡＴ活性的诱导。
In order to determine whether the low-affinity glucocorticoid binding
sites(LAG)mediates the bio-logical effects of high concentrations of glucocorticoids,we studied
the relationship between hydrocortisone(F)concentration and the induction of tyrosine
aminotransferase(TAT)activity in primary cultures of rathepatocytes.When the concentrations of
F were 1-10 nmol/L,the dose-response relationship showed atendency to saturation.The TAT
activity was much higher when the F concentration was over 10 nmol/L(up to 10μmol/L),and the
induction of TAT by 10 μmol/L F was completely inhibited by RU486,thecompetitive antagonist
of glucocorticoid receptor and LAGS.These results indicate that induction of TATby high
concentrations of F is mediated by LAG
出处
《第一军医大学学报》
CSCD
1994年第2期122-123,共2页
Journal of First Military Medical University
关键词
糖皮质激素
受体
肝
酪氨酸转氨酶
Glucocorticoid
receptor
mediation
liver
enzyme
rat
