摘要
分析经γ射线离体照射的正常人外周血单个核细胞（ＰＢＭＣ）及急性放射病恢复期患者ＰＢＭＣ中Ｔ细胞受体（ＴＣＲ）、ＣＤ_３、ＣＤ_（１８）及ＣＤ_（２５）阳性细胞。发现经１～６Ｇｙγ射线照射后立即分析，正常人上述４种标志阳性Ｔ细胞百分率无明显改变；受照射细胞经体外诱导培养４～１４ｄ，ＴＣＲ、ＣＤ_３及ＣＤ_（２５）阳性细胞百分率不同程度呈照射剂量依赖性下降，随培养时间延长增加，ＣＤ_（１８）阳性细胞百分率的变化幅度相对较小。免疫细胞化学分析显示，照射后８～１２ｄ，Ｔ细胞胞浆内有较多ＴＣＲ（β）及ＣＤ_３阳性物质出现。提示：辐射致ＴＣＲ、ＣＤ_３阳性Ｔ细胞减少可能与细胞内ＴＣＲ及ＣＤ_３表达和装配异常有关。
Using a flow cytometry(FCM),we detected and enumerated the positive cell percentage of T cell receptor(TCRα/β),CD_3,CD_(18)and CD_(25) in normal human peripheral blood mononuclear cells(PBMC)irradiated by γ ray in vitro and PBMC from persons who suffered acute radiation symptoms 3 years earlier.The results showed that after PBMC from the normal persons were irradiated with 1～6 Gy dose the positive cell percentage of TCR,CD_3,CD_(18)and CD_(25) did not change significantly when analysis was conducted immediately after PBMC were irradiated,while the positive cell percentage of TCR,CD_3 and CD_(25) decreased with the increase in irradiation dose and increased with prolongation of culture time significantly at different levels.The change in positive cell percentage of CD_(18) was relatively smaller than that of the others after irradiated PBMC were cultured in virto for 8～14d. Immunocytochemical analysis showed that more TCR and CD_3 positive stains presented in cytoplasm 8～12d after irradiation.These results implied that the decrement of positive TCR and CD_3 T cells induced by irradiation might be related to abnormal expression and disabled assembly of TCR/CD_3 complex.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1994年第5期417-422,共6页
Academic Journal of Second Military Medical University
