摘要
用体外短期液体培养技术，观察ＲＡ和不同来源的造血调控因子对ＨＬ－６０细胞增殖与分化的影响。以生长曲线和倍增时间作为观察各种试剂对ＨＬ－６０细胞增殖作用的指标；以ＮＢＴ还原反应和细胞形态变化作为分化成熟的指标。结果是ＲＡ、ＰＨＡ－ＬＣＭ单用时对ＨＬ－６０细胞均有抑制增殖和诱导分化作用，ＰＷＭ－ＳＣＭ、ＬＰ３－ＣＭ仅部分抑制增殖，而ＦＧＦ、Ｈｕ－ＣＳＦ和ＥＰＯ等无效。各种造血生长因子分别与ＲＡ合用时，能加强ＲＡ对ＨＬ－６０细胞的增殖抑制和诱导分化作用的有ＦＣＦ、ＰＷＭ－ＳＣＭ、ＰＨＡ－ＬＣＭ和Ｈｕ－ＣＳＦ等；ＬＰ３－ＣＭ仅增强抑制增殖：ＥＰＯ未显示作用。
Using a short-term liquid culture assay, the effects of induction of differentiation of HL-60 cells by retinoic acid (RA) and several hematopoietic modulation factors were evaluated.The results demonstrated that (1) RA and PHA-LCM were capable of inhibiting proliferation, and inducing differentiation of HL-60 cell line, respectively. (2) PWM-SCM and LP3-CM had partial an inhibitory effect on HL-60 cell growth while FGF, Hu-CSF and EPO had no such an effect.(3) although any one of hematopoietic modulation factors by itself had no siginficant effect on induction of differentiation of HL-60 cell line, it could euhance granulocytic maturation when used in combination with RA, except for LP3-CM and EPO.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1994年第2期123-129,共7页
Academic Journal of Second Military Medical University
基金
国家自然科学基金
关键词
HL-60
细胞系
诱导分化
白血病
hematopoietic modulation factor
retinoic acid
HL-60 cell line
induction of differentiation
