摘要
本文利用丙酮沉淀、凝胶过滤、离子交换层析等方法提纯了大鼠肝脏精氨酸酶,在SDS-PAGE中表现为单一的蛋白质带,其亚基分子量为37,000.建立了大鼠肝精氨酸酶的酶标免疫吸附测定法(ELISA),比较了两种常规的精氨酸酶活性测定法与ELISA法,并初步探讨了ELISA法在精氨酸酶测定方面的一些应用。
The rat liver arginase was purified by acetoneprecipitation, gel-filtration and ion-change chromatography. The purified arginase showed a single band in SDS-PAGE. The subunit M.W. was 37,000. We have established an enzyme linked im-munosorbent assay (ELISA) for rat liver arginase. comparison of this method with two commen methods of arginase activity assay was made. Applications of this method were also studied.
基金
国家自然科学基金
