摘要
应用基因重组技术，把Ｅｐｓｔｅｉｎ－Ｂａｒｒ（ＥＢ）病毒Ｔｒａｎｓ激活子ＢＺＬＦ１基因与原核载体ｐＥＸ１进行基因重组，构建成质粒ｐＥｘ１－ＢａｍＨＩＺ，并使ＢＺＬＦ１蛋白在大肠杆菌（Ｅ．ｃｏｌｉ）中高效表达，产物为１４１ｋＤ的ＬａｃＺ－ＢＺＬＦ１融合蛋白。经Ｗｅｓｔｅｍｂｌｏｔ证实，该融合蛋白可与兔抗ＢＺＬＦ１蛋白的多克隆抗体发生特异性反应。在所检测的５０份鼻咽癌病人血清中，ＩｇＧ／ＢＺＬＦ１抗体的检出率为９０％，而在正常人血清中则未查到此抗体。实验还证实，鼻咽癌病人血清中的抗ＥＢ病毒ＩｇＡ／ＥＡ和ＩｇＧ／ＢＺＬＦ１抗体有密切的相关性。
pstein-Barr virus (EBV), a human herpesvirus, is the causative agent of infectiousmononucleosis and is associated with Burkitt’s lymphoma, opportunistic lymphoma and masopharyngeal carcinoma (NPC). Usually, EBV can latently infect its target cells, but under some conditions, the latent EBV genome can also be activated by various agents,especially byits own trans activator BZLF1. The prokaryotic expressive plasmid pEX1-BamHI Z encoding the EBV trans activatol,BZLF1 gene, was successfully constructed. The fusion protein,LacZ-BZLF1,was expressed verywell in E. colipop2136.The molecular weight of this fusion plotein was shown to be 141kd(pZ141). By immunoblotting, the pZ141 could specially react with both rabbit polyclonal antibodiesagainst EBV BZLF1 protein, and antibodies in the sera from the patients with NPC,but notwith unrelated tabbit antibodies and antibodies in the seta from the normal indiiduals.IgG/BZLF1 antibodies were detected in the sera from 50 NPC patients and 55 normalindividuals. The positive rates were 90% and 0 respectively. The results also show that thereis a good relationship between the anti-EBV EA/IgA and BZLF1/IgG antibodies in the serafrom NPC patients.
出处
《病毒学报》
CAS
CSCD
北大核心
1994年第1期14-18,共5页
Chinese Journal of Virology
关键词
基因表达
E-B病毒
鼻炎肿瘤
Epstein-Barr virus, Nasopharyngeal careinoma,Plasmid pEX1-BamHI Z
