摘要
以获得的抗ＨＭＧ＿（１４）ＭｃＡｂ为探针，运用间接荧光免疫技术，对果蝇（Ｄ，Ｖｉｖｉｌｉｓ南京）唾腺染色体上ＨＭＧ＿（１４）的分布及其ＨＭＧ＿（１４）与活性区“ｐｕｆｆ”的关系进行了初步观察。发现；（１）ＨＭＣ＿（１４）分布在唾腺染色体所有的横纹带上；（２）在“ｐｕｆｆ”区，ＨＭＧ＿（１４）有增加的趋势；（３）ＤＮａｓｅＩ处理可以使染色体上的ＨＭＧ＿（１４）丢失。实验结果进一步验证了ＨＭＧ＿（１４）是染色体上的一种结构性蛋白。
A technique has been developed for "staining" cytological preparation by indirectimmunofluorescent methods that Permits determination of the in situ disiribution ofchromosomal proteins. The method is Particularly oriented to use of polytene chromosomesqnashes from Drosophila salivary gland. The results obtained demonstrate the specificin vivo chromosomal locations of nonhiston protein HMG purified froiri isolated chroma-tin. The fluorescent patterns that we have observed are: (1) All bands are stained, butquantitative differences in the fluorescence of particular bands are apparent. (2) Mostpuffs are brighty stained. The chromocenter is brightly stained. (3) After DNase Idigested, all fluorescent bands are losted. ( 4 ) The traditional technique yields the samequalitative fluorescent patterns.
出处
《北京大学学报（自然科学版）》
CSCD
北大核心
1994年第2期245-249,共5页
Acta Scientiarum Naturalium Universitatis Pekinensis
关键词
HMG14
唾腺
染色体
原位分布
HMG nonhiston protein
Drosophilai Polytene chromosomel Indirectimmunofluorescence
McAb
