摘要
应用多效唑((PP_(333))叶面喷洒能有效地抑制大叶黄杨新梢的生长。处理以后,植株节间缩短,节数稍减少,茎变粗,茎上部节密集,叶呈簇生状。抑制效果因处理浓度的增加而提高,因而可以保持修剪时的观赏造型。经多效唑处理的植株,其顶端分生组织原套及原体的结构排列不匀,该组织细胞染色呈不正常,细胞质、核染色加深;生物化学反应为植株叶片和茎尖中过氧化物酶的活性明显地受到影响。经多效唑400ppm处理的植株叶片和茎尖过氧化物酶活性迅速得到增加;2000ppm处理的植株,在叶片中增加了1条过氧化物酶同工酶谱带;以1000 ppm和2000ppm处理的植株茎尖中分别增加了2条和4条酶谱。凝胶扫描结果表明,新出现的过氧化物酶同工酶活性较低。处理植株酶活性的变化,主要表明在泳动率最大的那组同工酶活性的变化。
<Abstract > Foliar spray of Paclobutrazol solution resulted in reduction of shoot elongation of Evergreen Euonymus. This inhibitive effect increased with increase of the concentrations of spray solution. Treatments with 2000 and 4000 ppm Paclobutrazol inhibited shoot elongation almost completely and thus main- tained the initial ornamental shape of the plants. Plants treated had shorter internodes, less number of nodes, bigger shoot diameters and thicker leaves, so that the initial ornamental shape of plants would be maintained for a long period. Cytohistological observation showed that the normal cytohistological zona- tion of the meristems of plants treated was less distinct, and cells of the meris- tematic tissue were densely stained than those in control. Paclobutrazol treatment also affected the peroxidase activity in 1eaves and shoot tips of Evergreen Euonymus. Changes of enzyme activity were dependent on the cocentrations of Paclobutrazol solution and growth stage of plants. In treatment with 4000 ppm of Paclobutrazol, the peroxidase activity in both leaves and shoot tips of plants increased rapidly during a long period, and then, decreased with growth of shoots. 2000 ppm Paclobutraszol treatment induced ap- pearance of one peroxidase isozyme in leaves. While 1000 and 2000 ppm Paclobu- trazol treatments induced appearance of two and four peroxidase isozymes, respectively in the shoot tips of plants.
出处
《上海农业学报》
CSCD
1989年第1期47-54,共8页
Acta Agriculturae Shanghai
关键词
大叶贡杨
多效唑
株型控制
Euongmus Juponica
Paclobutrazol
Control of plant shape
Physiological effect
