摘要
本文采用放线菌酮处理的McCoy细胞分离培养泌尿生殖道沙眼衣原体。450例宫颈炎,盆腔炎患者培养阳性234人(52%),30例男性尿道炎患者培养阳性15人(50％),提示沙眼衣原体引起的泌尿生殖道感染在国内十分常见。249份阳性中二次传代阳性73人(29.3％),放线菌酮最适浓度为1～4μg/ml,经离心处理的标本包涵体形成数明显高于不离心标本。用放线菌酮处理细胞、离心。
Chlamydia trachomatis in genitourinary tract were cultivated and isolated by culturing McCoy cells treated with cycloheximide. Of the 450 cases of cervicitis Or inflammatory diseases,,234(52%) positive cultures were detected. 15(50%) positive cultures were detected in 30 cases of urethritis in men. It was suggested that genitourinary infacfions caused by C.trachomatis were common in our country. Of 249 positive cultures, 73(29.3%) were detected by the second passage. Cycloheximide optimal concentration in inoculation media was 1-4ng/ml. Inclusion number of the samples centrifugad at 2000g was higher than that of uncentrifuged samples. Cells treated with cycloheximide,cefltrifugation, and a second passage were necessary for cultivation and isolation of the pathogen
出处
《重庆医科大学学报》
CAS
CSCD
1993年第3期229-232,共4页
Journal of Chongqing Medical University
基金
四川省卫生厅资助课题
关键词
沙眼衣原体
感染
泌尿生殖系统
C. traehomatis, C. trachomatis cultures
genitourinary infection
