摘要
13种人工合成HIV多肽经ELISA检测发现,CH-10抗原性最强,其次为CH-10a和CH-12a。其余10种抗原性较弱。抗原浓度以80μg/ml为宜。CH-10与Organon试剂盒比较,结果基本相似。初步实验证明合成肽可望替代病毒抗原或重组抗原,用于血清HIV抗体检测.
Antigenicity of 13 synthetic HIV peptides were tested by ELISA. The results indicated that CH-10 was the most antigenitic peptide as compared with CH-10a and CH-12a. An 80 μg/ml concentration of HIV peptide was best. Similarities were found by comparing CH-10 peptide with the Organon test kit. The results showed that synthetic HIV-peptide may be a substitute for HIV virus or recombinant HIV antigen for the detection of HIV-antibody in serum.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1993年第2期128-131,共4页
Acta Academiae Medicinae Sinicae
关键词
人工合成
多肽
艾滋病毒
synthetic peptides HIV antigenicity ELISA
