摘要
目的 研究大鼠提睾肌神经损伤后血管平滑肌细胞和内皮细胞的Bcl -2、Bax及P53 表达和意义。方法以免疫组织化学SP法 ,对大鼠提睾肌神经损伤后的血管平滑肌细胞和内皮细胞的Bcl -2、Bax及P53 表达进行研究。结果 支配提睾肌的神经损伤后 ,Bcl-2、Bax和P53 均有表达。在平滑肌细胞中 ,Bcl -2的表达明显强于Bax和P53,而在内皮细胞Bax和P53 的表达强于Bcl-2。结论 大鼠提睾肌损伤后平滑肌细胞有增殖现象 ,而内皮细胞则可能出现过度凋亡 ,上述改变可能对微循环产生不利影响。
Objective To study the expression of Bcl-2,Bax and P 53 and its significance in vascular smooth muscle cells and endothelial cells with injured cremaster nerve of the rat.Methods Immunohistochemical SP method was used in this study.Results The expression of Bcl-2,Bax and P 53 in smooth muscle cells and endothelial cells was obvious but the number of positive cells of Bcl-2 was larger than that of Bax and P 53 in smooth muscle cells.In endothelial cells,expression of Bax and P53 maintained at a higher level.Conclusion Cremaster nerve injury could result in proliferation of smooth muscle cells and apoptosis of endothelial cells and might play an important role in dysfunction of microcirculation.
出处
《中国实验动物学杂志》
2001年第1期19-22,共4页
Chinese Journal of Laboratory Animal Science
