摘要
利用抗药性标记基因(Hygromycin B:hygB)建立稻瘟病菌的转化系统。结果表明,Aspergillus的TrpC转录信号与细菌的hygB抗性结构基因重组的质粒pCSN43可以在稻瘟病菌中表达,该质粒与受体菌无同源顺序,因而有利于对转化菌株进行筛选和分析。电激法和PEG/Ca^(2+)法均适用于转化稻瘟病菌,但后者的转化效率比前者高。
A transformation system based on a dominant selectable marker, hygromycin B resistance (hyg B′) gene, was established for Pyricularia oryzae. The plasmid pCSN43 conlaining a hyg B′ structure gene with a Trp C transcription signal of Aspergillus was functional in P. oryzae. There are neither homologous sequences of the marker in the recipient genome nor any hyg B′ spontaneous mutants. The transformation system offerred a clean background and facilitated selection and identification of transformants. Both electroporation and PEG/Ca^(2+) methods worked for transformation, and the high est transformation frequency was 25 transformants per microgram vector DNA on PEG/Ca^(2+) method.
出处
《中国水稻科学》
CAS
CSCD
北大核心
1993年第4期232-238,共7页
Chinese Journal of Rice Science
关键词
显性标记基因
稻瘟病
转化
水稻
Dominant selectable marker
Hygromycin B resistance gene
Pyricularia oryzae
Transformation
