摘要
超薄切片观察云南烟草丛枝症病害的病叶组织 ,并抽提试验材料的总核酸电泳检测。病组织中检测到与烟草丛枝症病害相关的低分子量RNA ,切片中未观察到病毒粒子和植原体等病原 ,薄壁细胞中发现细胞膜系统的畸变 :质膜体和细胞质泡囊 ,质膜体 (1 0 0 0～ 2 0 0 0nm)结构类似于病毒形成的旁壁体 ,细胞质泡囊(1 0 0～ 2 0 0nm)是含纤维状物的双膜的囊。运用不同盐浓度介质的RNase处理病变细胞组织 ,发现高盐介质的Rnase (2XSSX)不能消解小囊泡中的纤维状物 ,而低盐介质的Rnase (0 0 1XSSC)可以消解小囊泡中的纤维状物。文献显示这些病变是类病毒和某些病毒侵染的特征 ,初步认为这是云南烟草丛枝症病害发病早期细胞内出现小分子核酸侵染的细胞病变特征。
Leaves of Tobacco withches' broom symptom disease in Yunnan from diseased plants(detected the Tobacco witches' broom symptom diseases associated-RNA) and healthy plants were fixed and processed for transmission electron microscopy.No virus-like and phytoplasmas were found in diseased thin sections.Aberration of the plasmamenbrane of campanion cells have been identified:the plamalemmasome and cytoplasmic vesicles,The plamalemmasome (with diameter ranged 1000～2000nm) structure likes viroid-associated plamalemmasome,and cytoplasmic vesicles (with diameter ranged 100～200nm)were double membranous vesicles concluding fibrous thing.The vesicles were different from phytoplasma in distributing.The fibrils in vesicles of diseased tobacco leaf cells can be digested with Rnase in low medium(0.01XSSC).which can't be digested in high medium(2XSSC).According to some documents,these aberratons were the characteristics of the viroid or virus infected leaf tissue.We think that perhaps the pathogen of the disease ist't phytoplasma preliminarily.
出处
《山东农业大学学报（自然科学版）》
CSCD
2000年第3期269-272,共4页
Journal of Shandong Agricultural University：Natural Science Edition
基金
云南省应用基础研究基金
云南省烟草科研基金联合资助重点项目
关键词
草丛
病害
病变
细胞质
质膜
双膜
超微细胞
观察
细胞内
发病
tobacco witches broom symptom diseases
ultrastructure
plamalemmasome cytoplasmic vesicles
