摘要
本文采用聚合酶链反应技术对爪哇型和帕托克型两株钩端螺旋体(简称钩体)的部分16s rRNA基因进行扩增和核苷酸序列分析,并与已报道的犬型钩体序列加以比较。结果发现爪哇型钩体与犬型钩体的16s rRNA基因核苷酸序列具有高度同源性,而在爪哇型与帕托克型之间则存在着较明显差别。
In this study , the partial 16S ribosomal RNA Genes of two strains of leptospira(serovar Javanica and serovar Patoc)were amplified and sequenced.by polymerase chain reaction,compared with that of serovar Canicola which was reported previously. The results showed that the 16S rRNA genes sequence in pathogenic leptospirae was in high homology,but there was an obvious difference between pathogenic leptospirae and L. biflexa.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1993年第2期7-10,共4页
Chinese Journal of Zoonoses
关键词
钩端螺旋体
RRNA
核苷酸
基因
Leptospira,Ribosomal RNA ,Polymerase chain reaction.
