摘要
观察自发性高胆固醇血症近交系ＮＪＳ小鼠的主动脉形态学特征，了解该品系主动脉粥样硬化发病情况。对主动脉作光镜和电镜观察，并通过图像分析仪分析主动脉内膜的粥样硬化病变面积。该品系８～１０月龄小鼠均见阳性病灶，阳性区域主要分布在动脉弓起始部，胸主动脉各分布的升口处，病变区域多数呈点状，少量呈线状分布。显微镜下内膜层内可观察到少数泡沫细胞聚集，电镜下可见内膜泡沫细胞有较多的细胞突起，胞浆丰富，浆内内质网及溶酶体丰富，脂滴大小不一，核形态不规则，浆内有明确肌丝或致密斑。图像分析计算其主动脉粥样硬化病变面积占主动脉面积的比例为２．０４６±０．２５３％。２～３月龄小鼠中均未见明显病变。近义系ＮＪＳ小鼠８月龄后主动脉能发生脂纹期病变，该品系可作为动脉粥样硬化研究的动物模型。
Aim This study is to observe morphological features on aorta of inbred NJS mouse strain with spontaneoous hypercholesteroemia,and to investigate pathological changes of atherosclerosis of the strain. Methods Histological,electromicroscopic examination and computer aid image analysis were used in the study. Results The incidence of atheroscerosis of the strain was 100% in some liable positions such as arotic sac and ascending aorta at 8～10 months.It appeared stripy or flaky obviously on some red coloring positions by staining.There were some foamy cell in aortic wall by tissue flat staining.No pathological changes were seen at 2～3 months of age. Conculsion Inbred NJS mouse strain can develop earily lesions after 8 months of age and can be used as a modle on research of pathogenesis in atheroscerosis.
出处
《中国实验动物学报》
CAS
CSCD
1997年第1期61-64,共4页
Acta Laboratorium Animalis Scientia Sinica
基金
"九五"军队医药卫生科研基金
