摘要
目的 探讨应用胃泌素受体拮抗剂治疗胃癌的可行性。方法 在体外条件下观察胃泌素及其受体拮抗剂丙谷胺对胃癌细胞株ＭＫＮ４５ 的细胞活力、细胞内ｃＡＭＰ浓度及细胞凋亡的影响；在体内条件下观察胃泌素受体拮抗剂丙谷胺对胃癌细胞株ＭＫＮ４５ 移植瘤的面积、重量及胃癌细胞ＤＮＡ 的影响。结果 在体外条件下，胃泌素能促进胃癌细胞株ＭＫＮ４５ 的增殖，细胞内ｃＡＭＰ浓度增高，细胞凋亡百分率降低，而丙谷胺能完全阻断胃泌素的作用。在体内条件下，丙谷胺能抑制胃癌细胞株ＭＫＮ４５ 移植瘤的生长，肿瘤面积、重量及胃癌细胞ＤＩ、ＤＮＡ 含量、ＳＰＦ均低于对照组。结论 胃泌素受体拮抗剂能够抑制胃泌素受体阳性的胃癌细胞生长，有望为胃癌的治疗提供一种新手段。
Objective To evaluatetheeffect of a gastrin receptorantagonistin thetreatment of gastric cancer.Methods The level of cell vitality ,intracellular cAMPconcentration and cell apoptosisrate of gastric cancer cells MKN45 was measured in vitro under the use of gastrin and gastrin receptor antagonist proglumide respectively .The xenotransplanted tumor areas, weight and the DNAcontent of gastric cancer cells MKN45 in vivo was observed under the use of proglumide. Results In vitro ,gastrin promoted the cell proliferation of gastric cancer cells MKN45 with higher intracellular cAMPlevel and lower cell apoptosis rate . Proglumide could interrupt the effect of gastrin .In vivo ,proglumideinhibited the growth of xenotransplanted gastric cancer cells MKN45 . Tumor area, weight, DNAindex ,DNAcontentand Sphasefraction of gastric cancer waslowerin proglumide group than thatin control group . Conclusion Gastrin receptor antagonist can inhibit the growth of gastric cancer with positive gastrin receptor, which may offer a new way in the treatment of gastric cancer.
