摘要
目的使用免疫抑制法检测α-淀粉酶同工酶胰腺淀粉酶（P-AMY），以评价其诊断急性胰腺炎的临床价值。方法对55例急性胰腺炎，81例急性胆囊炎的血清和尿液α-AMY总活性及P-AMY活性和P／α比值进行了对比研究。结果血清和尿液P-AMY的参考范围为6-46U／L和58-398U／L，急性胰腺炎组血清α.P-AMY.P／α及尿液α.P-AMY均较急性胆囊炎组病人有高度显著性差异（P＜0.01），但两组病人尿液P／α比值无显著性差异（P＞0.05）。急性胆囊炎病人血清α.P-AMY，P／α及尿液α.P-AMY校正常健康人无显著性差异（P＞0.05），但两组病人尿液P／α比值有显著性差异（P＜0.05）。在136例病人中，急性胰腺炎病人血清P-AMY的诊断特异性效率最高，分别为92.6％和89％。结论该方法检测P-AMY准确，特异，快速，可作为急性胰腺炎的诊断和鉴别诊断的重要依据。
To determine α -amylase iasenzwne pancreatic α-amylase (P - AMY)by irnxnuncrinhibition method and to evaluate its clinical value in the diagnosis of acute pancreatitis. Method Total activity of α - AMY, activity of P - AMY and P/α ratio in the serum and urine of 55 patients with acute pancreatitis and 81 with acute cholecystitis were studied and compared. Result The reference ranges of P - AMY in the serurn and urine were 6 - 46U/L and 58 - 398U/L respectively. There was significant difference in serum. P - AMY- P/α ratio and urine α- P - AMY between the acute pancreatitis group and acute cholecystitis(P< 0. 01 )group, but not in P/α ratio in the urine between the two groups(P>0. 05). There was no significant difference in serum. α. P -AMY, P/α ratio and urino α. P - AMY between the acute cholecystitis group and norml group(P>0.05), but the urine P/α of the two groups showed significant difference. In 136 patients with acute pancreatitis the diagnostic specificity and effciency of serum P-AMY were highest, being 92. 6% and 89% respectively. Conclusion This method is accurate. specific and rapid to detect serum P - AMY and can be uSed as an important basis in the diagnosis and differential diagnosis of acute pancreatitis.
出处
《江西医学检验》
1998年第2期64-66,共3页
Jiangxi Journal of Medical Laboratory Sciences
关键词
Α-淀粉酶
胰腺淀粉酶
单克隆抗体
免疫抑制法
Alpha-amylase Pancreatic alpha-amylase Monoclonal-antibody Immuno-inhibition method
