摘要
氧化修饰低密度脂蛋白(Ox—LDL)在动脉粥样硬化发生发展中的作用越来越引起人们的重视。本文通过比较Ox—LDL和脂质过氧化降解产物丙二醛(MDA)修饰的低密度脂蛋白(MDA—LDL)在致泡沫细胞形成方面的差异,探讨了脂质过氧化对巨噬细胞的损伤在泡沫细胞形成中的作用。结果显示:Ox—LDL和MDA—LDL都可被巨噬细胞清道夫受体所识别,引起大量吞噬,造成细胞内胆固醇的聚集,但由MDA—LDL造成的细胞内胆固醇酯聚集可被高密度脂蛋白(HDL_3)所清除,而Ox—LDL造成的胆固醇酯聚集则不能。进一步的研究表明Ox—LDL和MDA—LDL对巨噬细胞HDL_3结合量及细胞内脂质过氧化物(LPO)含量的影响不同。虽然MDA—LDL和Ox—LDL处理巨噬细胞,都可使其HDL_3结合量有不同程度的下降,细胞内LPO含量有不同程度的升高,但当处理因素消除后,细胞继续培养时,由MDA—LDL处理的细胞其降低的HDL_3结合量又有一定的恢复,细胞LPO含量不再上升;而由Ox—LDL处理的细胞,其HDL_3结合量则继续下降,细胞LPO含量则继续升高。由Ox—LDL导致的巨噬细胞HDL_3结合量下降与细胞LPO含量升高之间呈负相关(r=-0.81,P<0.01)。用叔丁基脂氢过氧化物(tbooh)(1×10^(?)mol/L)对巨噬细胞损伤24小时,然后用两种修饰的LDL处理,则两种修饰LDL造成的胆固醇酯聚集都不能被HDL_3清除。本文结果提示Ox—LDL对巨噬细胞的脂质过氧化损伤可能在巨噬细胞向泡沫细胞转变过程中起着重要作用。
Both oxidatively and malondialdehyde mosified low density lipoprotein (Ox—LDL and MDA—LDL)could be recongized by the scavenger receptor and induce intracellular cholesteryl ester accumulation of macrophage. The cholesteryl ester accumulation caused by MDA—LDL could be cleared by high density lipoprotein (HDL_3),but that caused by Ox—LDL could not be cleared. Further studies showed that both Ox—LDL and MDA—LDL could decrease the binding capacity of HDL_3 and increase the intracellular lipid peroxide (LPO). When macrophage was first cultured with MDA—LDL and then in medium containing 10% (v/v) lipoprotein deficent serum, the decreased binding capacity of HDL_3 was somewhat recovered and the intracellular LPO did not increase any more. However,if macrophage was first cultured with Ox—LDL,the binding capacity of HDL_3 continued to decrease and intracellular LPO continued to increase. There was negative correlation (r=-0.81, p<0.01) between the decrease of the binding capacity of HDL_3 and the increase of intracellular LPO caused by Ox—LDL. These results suggested that lipid peroxidative injury of Ox—LDL may play an important role in the transformation of macrophages to foam cells.
出处
《中国动脉硬化杂志》
CAS
CSCD
1993年第1期30-35,共6页
Chinese Journal of Arteriosclerosis
关键词
低密度脂蛋白
氧化
动脉粥样硬化
low density lipoprotein
oxidative modification
malondialdehyde modification
high density lipoprotein 3
foam ceil
maerophage
