摘要
本实验利用BALB/C裸小鼠,复制荷人鼻咽癌(nasopharyngeal carcinoma,NPC)裸鼠模型,检测荷瘤鼠血浆丙二醛(malondialdehyde,MDA)、cAMP、cGMP和cAMP/cGMP比值,观察云芝糖肽(polysacchoridopetide,PSP)对荷人NPC裸鼠的抑瘤作用及对荷瘤鼠血浆MDA和cAMP/cGMP等的影响。结果发现荷瘤鼠血浆MDA升高,cAMP、cGMP降低,cAMP/cGMP比值升高,高低浓度PSP对荷人NPC裸鼠均有明显抗癌作用(P<0.01),抑瘤率59.58～95.53%;并可降低荷瘤鼠血浆MDA,提高血浆cAMP、cGMP水平,恢复cAMP/cGMP比值。本实验提示,荷瘤鼠血浆cAMP等降低可能与MDA升高有关。PSP对荷人NPC裸鼠有抑瘤作用,其作用机理可能是通过降低荷瘤鼠血浆MDA,恢复cAMP/cGMP比值。
Nasopharyngeal carcinoma (NPC) in BALB/c nude mice (NM) was re-produced by hypodermic implanting human CNE-2 cell to the right-side of neck-backposi-tion. The changes of plasma malondialdehyde (MDA) and cAMP/cGMP ratio in NM bea-ring human NPC were examined. Meanwhile, we observed that tumor's size, plasma MDAand cAMP/cGMP ratio in NM bearing human NPC were affected by polysacchoridopetide(PSP). Results indicated that in comparison with the control group, NM bearing humanNPC showed a marked increase in plasma MDA(18.25±1.37 nmol/ml),cAMP/cGMP ratio(4.55±0.35), a marked decrease in plasma, cAMP (71.83±8.51 pM/ml) and cGMP(15.77±0.65 pM/ml), both high or low-concentration PSP inhibited NPC growth.Rate of inhibition was between 59.58 to 95.53 percent. PSP decreased plasma MDA,elevated cAMP, cGMP's levels and recovered cAMP/cGMP ratio. Our results suggestedthat the elevated level of MDA in NM bearing human NPC was closely associated withthe presence of NPC. PSP had antitumor effect, probably by means of decreasing freeradicals and recovering cAMP/cGMP ratio.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1993年第5期600-603,共4页
Chinese Journal of Pathophysiology
关键词
鼻咽肿瘤
游离基类
多糖肽类
小鼠
Nasopharyngeal neoplasms
Free radicals
Adenosine cyclic monophosphate
Polysacchorides
Mice,nude
