摘要
本研究用PAP法、胸腺细胞增殖法、脾细胞增殖法,分别检测16例体外HBV感染的骨髓单个核细胞与16例慢性乙型肝炎患者体内感染的骨髓单个核细胞(MNCs)中的HBcAg和白细胞介素-1(IL-1)、白细胞介素-2(IL-2)的诱生活性(以△cpm值表示)。结果显示,体外HBV感染组与体内HBV感染组骨髓MNCs中HBcAg检出率分别为50％和43.7％。本实验结果表明,HBV在体外感染骨髓MNCs,且与体内自然感染相符,但光镜下未观察到致细胞病变效应(CPE)。体外感染组与体内感染组IL-I和IL-2活性均较对照组明显下降(P<0.01)。且细胞中HBcAg检出阳性者较阴性者下降更为明显(P<0.01)。IL-1和IL-2诱生活性降低与HBV侵染免疫细胞及其在细胞内复制有密切关系,从而提示,IL-1和IL-2降低可能影响HBV的清除而引起慢性化过程。
The levels of interleukin-1 and interleukin-2 production in 16 healthy blood donors' bone mar row mononuclear cells infected by HBV in vitro and on 16 cases with chronic hepatitis B were simulta neously assayed. The evidence of virus growth is based on the detecion of HBcAg in bone marrow mononuclear cells by immunoperoxidase assay. It was found that HBcAg is positive in 50% (in vitro) and 43. 7％(in vivo) respectively (P<0.05). The levels of interleukin-1 and interleukin-2 produetion are markedly decreased in both HBV infected groups compared with controls (p<0.01), and Inter leukin-1 and interleukin-2 decreased more significantly in HBcAg positive cases than that of HBcAg negative eases(P<0.01). There was highly significant eorrelation(P<0.001)between levels of inter leukin-1 and interleukin-2. The results suggest that HBV can infect and replicate in human bone mar row mononuelear cells in vitro and in vivo. The decrease of interleukin-1 and interleukin-2 level may be closely related to the defficulty in HBV elimination.
出处
《中国病毒学》
CSCD
1993年第4期332-337,共6页
Virologica Sinica
关键词
乙型肝炎病毒
单核细胞
白细胞介素
Interleukin-1
Interleukin-2
Bone marrow mononuclear cell
Hepatitis B virus
Replication
