摘要
研究了蛋白质化学交联剂二氟二硝基苯对叶绿体类囊体膜功能的效应。检测到ＤＦＤＮＢ抑制光合磷酸化，降低光照诱导类囊体膜的质子吸收和９－氨基吖啶（９－ＡＡ）的荧光猝灭，降低电色效应的快相上升，显著抑制膜上腺三磷酶的活性，ＤＦＤＮＢ和游离的腺三磷酶进行交联反应，再经ＳＤＳ－聚丙烯酰胺凝胶电泳表明，交联后的酶出现新的蛋白染色带。
The effects of crosslinking agent-DFDNB (difluoro dinitro benzene) on functions of chloroplast thylakoid membrane proteins were investigated. DFDNB inhibited activites of PSP and membrane-bound ATPase in chloroplasts. It decreased proton uptake of light-inducted chloroplast thylakoids and the relative value of fluorescence quenching of 9-aminoacri-dine, and inhibited the rate of fast electrogenic phase of absorption change at 515 nm in chloroplasts. In addition, the isolated CF_1-ATPase was crosslinked with DFDNB. The pattern of polymers of crosslinked CF_1-ATPase was observed on SDS-PAGE.
基金
国家自然科学基金资助课题
关键词
类囊体
叶绿体
蛋白
化学交联作用
DFDNB(difluore dinitro benzene)
Thylakoids
Protein
Energy-trasduc- ing reaction
Crosslinking
